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Effects of lead (Pb) exposure on some blood biochemical indices in Cyprinus carpio: potential alleviative effects of thiamine

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Abstract

The aim of the present work was to study the alleviative properties of thiamine-supplemented fish diet on serum levels of Pb and some biochemical alterations provoked by Pb intoxication in carp. Fish in group 1 formed the untreated control group while fish in groups 2 and 3 received lead acetate (5 ppm) for 15 days. Group 3 was administered with thiamine (50 mg/kg diet) during lead exposure. The results revealed significantly raised levels of circulating Pb and malondialdehyde in group 2 compared to the control group. Significant decreases in circulating Pb and malondialdehyde contents in group 3 were observed following thiamine administration, as compared to group 2. Plasma values of alanine aminotransferase (ALT), urea, creatinine, and uric acid increased in group 2 compared to the control group, although the increase was only significant (p < 0.05) for ALT and creatinine. Thiamine supplementation effectively decreased ALT and creatinine concentrations to the levels observed in the apparently healthy controls. The other plasma biochemical indices quantified did not exhibit any significant modifications among experimental groups. These results are indicative of some variations caused by Pb intoxication in some plasma indices which signify the toxicity of Pb in Cyprinus carpio. Moreover, co-administration of thiamine with Pb notably reversed the expression of circulating biochemical biomarkers provoked by lead poisoning.

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Funding

This work was supported by a grant (No. 21603) from Ferdowsi University of Mashhad, Mashhad, Iran.

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Authors and Affiliations

  1. Department of Food Hygiene and Aquaculture, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

    Kimiya Nourian & Davar Shahsavani

  2. Department of Basic Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

    Hasan Baghshani

Authors
  1. Kimiya Nourian
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  2. Davar Shahsavani
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  3. Hasan Baghshani
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Correspondence to Davar Shahsavani.

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Ethical approval

The experiment protocol was in compliance with the advices of Research Ethics Committee of Ferdowsi University of Mashhad.

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The authors declare that they have no conflict of interest.

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Nourian, K., Shahsavani, D. & Baghshani, H. Effects of lead (Pb) exposure on some blood biochemical indices in Cyprinus carpio: potential alleviative effects of thiamine. Comp Clin Pathol 28, 189–194 (2019). https://doi.org/10.1007/s00580-018-2814-2

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  • DOI: https://doi.org/10.1007/s00580-018-2814-2

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